Refusal by Britain's prime minister to discuss an independence referendum would "shatter beyond repair" the United Kingdom's constitutional structure, Nicola Sturgeon told her Scottish National Party on Saturday.
Sturgeon, Scotland's First Minister, pledged to press on with plans to hold a new Scottish referendum as announced earlier this week, deepening a standoff with the UK government.
Party faithful cheered, clapped and leapt to their feet.
Sturgeon expects to get authorization from the devolved Scottish parliament on Wednesday to seek the terms for a new secession vote, aiming for a date once the terms for Brexit are clear but before Britain leaves the EU.
"To stand in defiance of (Scottish parliamentary authorization) would be for the prime minister to shatter beyond repair any notion of the UK as a respectful partnership of equals," Sturgeon said.
"Scotland's future will be in Scotland's hands."
Under the UK's constitutional arrangements, Britain's parliament needs to sign off on any legally binding vote in Scotland. Prime Minister Theresa May told Sturgeon this week that "now is not the time" for a new choice on independence as divorce talks between the world's fifth-largest economy and its erstwhile EU partners get under way.
Although May did not deny a vote outright, Scottish nationalists predict her words could build support for secession because she could be seen as telling Scotland what to do.
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"(May) has time to think again and I hope she does. If her concern is timing then — within reason — I am happy to have that discussion," Sturgeon said.
Britain is expected to trigger Article 50 of the EU's Lisbon Treaty within days and start the complex Brexit procedure.
Last June's vote to leave the EU has shaken the ties of the United Kingdom's four nations. England, the most populous, and Wales voted to leave while the Scots and Northern Irish wanted to keep their EU membership.